中医学报

目的:基于数据挖掘分析中医药治疗老年肌少症的用药规律，运用网络药理学探讨核心药物组治疗该病的作用机制。方法:以中国知网、万方数据、维普期刊全文数据、Pub Med等中英文数据库收录的相关文献为数据来源建立数据库，统计中药频次、性味和归经，基于频次分析、关联规则分析、聚类分析和复杂网络分析探讨用药规律，通过网络药理学探讨核心药物组治疗该疾病的作用机制。结果:共纳入处方40首，涉及中药117味，累计频次450次，高频药物依次为白术、甘草、茯苓、黄芪、党参等;药性以温、平为主;药味以甘、辛为主;归经以肝、脾、肾经药物为主;涉及10种中药类别。关联规则分析表明，白术-甘草提升度较高。聚类分析将高频药物分为3组。复杂网络分析确定核心药物组(白术、甘草、茯苓、党参、陈皮、黄芪)，其治疗老年肌少症涉及296种成分和118个潜在靶点。脂肪细胞因子、长寿调节途径、胰岛素抵抗等信号通路与核心药物组治疗老年肌少症密切相关。分子对接结果显示，关键活性成分维生素D3、大黄素、毛蕊异黄酮等与关键靶蛋白具有较高的结合活性。结论:老年肌少症的中医临床用药以脾为核心，注重气血双补、肝肾同调，兼顾活血化瘀。核心药物组可能通过多途径多靶点调节脂质代谢与炎症相关通路改善老年肌少症。

Objective: The purpose of this study is to analyze the medication patterns of Traditional Chinese Medicine(TCM) in the treatment of sarcopenia in the elderly using data mining,and to explore the mechanism of action of the core drug combination for sarcopenia through network pharmacology. Methods: A database was established using relevant literature sourced from Chinese and English databases such as CNKI,Wan Fang Data,VIP Journal Full-text Database and Pub Med. The drug frequency and flavor meridian analysis were orgenized. Medication patterns were investigated based on frequency analysis,association rule analysis,cluster analysis,and complex network analysis. Network pharmacology was applied to elucidate the mechanism of action of the core drug combination in treating sarcopenia. Results: A total of 40 prescriptions involving 117 Chinese medicinal herbs with a cumulative frequency of 450 were included. The high-frequency drugs were respectively Baizhu(Atractylodes macrocephala),Gancao(Glycyrrhizae Radix),Fuling(Poria),Huangqi(Astragali Radix),Dangshen(Codonopsis Radix). The majority of herbs were sweet and pungent in taste,warm and neutral in nature,and primarily associated with the Liver,Spleen,and Kidney meridians. Ten categories of herbs were involved. Association rule analysis revealed a high lift value between Baizhu(Atractylodes macrocephala) and Gancao(Glycyrrhizae Radix). Cluster analysis categorized high-frequency herbs into three groups,and complex network analysis identified a core drug combination comprising Baizhu(Atractylodes macrocephala),Gancao(Glycyrrhizae Radix),Fuling(Poria),Dangshen(Codonopsis Radix),Chenpi(Pericarpium Citri Reticulatae) and Huangqi(Astragali Radix). The core drug combination was found to involve 296 compounds and 118 potential targets in the treatment of sarcopenia in the elderly,with pathways such as adipocytokine signaling pathway,longevity regulating pathway,and insulin resistance being closely related. Molecular docking results showed that key active compounds like Vitamin D3,Emodin,and Calycosin had high binding activity with critical target proteins. Conclusion: The clinical use of TCM for sarcopenia in the elderly focuses on the Spleen as the core,emphasizing the simultaneous replenishment of qi and blood,as well as the harmonization of liver and kidney functions,while also attending to blood circulation and removing blood stasis. The core drug combination may ameliorate sarcopenia through regulating lipid metabolism and inflammation-related pathways via multiple pathways and targets.