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目的：探讨中医休粮疗法对肥胖型多囊卵巢综合征(polycystic ovary syndrome, PCOS)患者肠道菌群的影响。方法：选取2021年12月至2022年7月在江西中医药大学附属医院阳明路门诊肥胖与代谢病科完成中医休粮疗法的肥胖型PCOS患者为研究对象。比较7例患者在休粮前、休粮1个月和休粮3个月时的肠道菌群多样性变化及分布特征。结果：(1)Alpha多样性分析：与休粮前比较，患者休粮1个月后的Ace指数和Chao指数降低(P<0.05)。(2)菌落组成分析：在门水平方面，与休粮前比较，休粮1个月时厚壁菌门相对丰度减少，拟杆菌门、放线菌门、变形菌门和梭杆菌门丰度增加，差异均无统计学意义(P>0.05);休粮3个月与休粮前比较，厚壁菌门、放线菌门、变形菌门和梭杆菌门丰度减少，拟杆菌门丰度增加，差异均无统计学意义(P>0.05)。在属水平方面，与休粮前比较，休粮1个月和休粮3个月时拟杆菌属(Bacteroides)丰度显著增加(P<0.05),休粮1个月时罗氏菌属(Roseburia)丰度显著低于休粮前(P<0.05),休粮3个月时较休粮1个月时丰度增加(P<0.05)。(3)LEfSe分析：休粮前，组中糖单孢菌纲(Saccharimonadia)、糖单孢菌目(Saccharimonadales)、糖单孢菌科(Saccharimonadaceae)、Patescibacteria、TM7x、阿德尔克鲁茨菌属(Adlercreutzia)、罗氏菌属(Roseburia)、乳酸螺菌属(Lachnospira)、短杆真细菌群(Eubacterium＿brachy＿group)、阴性杆菌属(Negativibacillus)和真细菌肠道群(Eubacterium＿ventriosum＿group)在相对丰度方面具有优势；休粮1个月，组中拟杆菌属(Bacteroides)、拟杆菌科(Bacteroidaceae)、肠球菌科(Enterococcaceae)和肠球菌属(Enterococcus)在相对丰度方面具有优势。结论：中医休粮疗法对肥胖型PCOS患者的肠道菌群具有显著调节作用，可能通过影响短链脂肪酸的生成从而调节机体代谢。
Abstract：Objective: To explore the effect of traditional Chinese medicine fasting therapy on the gut microbiota of obese patients with polycystic ovary syndrome(PCOS).Method: Obese PCOS patients who completed traditional Chinese medicine fasting therapy at Yangming Road Outpatient Department of Jiangxi University of Chinese Medicine Affiliated Hospital from December 2021 to July 2022 were selected as the research subjects.Compare the changes and distribution characteristics of gut microbiota diversity among 7 subjects before, after 1 month, and after 3 months of fasting therapy.Results:(1) Alpha diversity analysis: Compared with before fasting therapy, the Ace index and Chao index of patients decreased after one month of fasting therapy(P<0.05).(2) Colony composition analysis: At the phylum level, compared with before fasting therapy, the relative abundance of Firmicutes decreased after one month of fasting therapy, while the abundance of Bacteroidetes, Actinobacteria, Proteobacteria, and Fusobacteria increased.The differences were not statistically significant(P>0.05);Compared with before fasting therapy for 3 months, the abundance of Firmicutes, Actinobacteria, Proteobacteria, and Clostridium decreased, while the abundance of Bacteroidetes increased, but the differences were not statistically significant(P>0.05).In terms of genus level, the abundance of Bacteroides significantly increased(P<0.05) after one month and three months of fasting therapy compared with that before fasting therapy.The abundance of Roseburia was significantly lower after one month of fasting therapy than before fasting therapy(P<0.05),and the abundance increased after three months of fasting therapy compared with one month of fasting therapy(P<0.05).(3) LEfSe analysis: Before fasting therapy, the Saccharimonadia, Saccharimonadaales, Saccharimonadaceae, Patescibacteria, TM7x, Adlercreutzia, Roseburia, Lachnospira, Eubacterium-brachy＿group, Negativibacillus, and Eubacterium-creditiosum-g groups in the group showed relative abundance advantages; After one month of fasting therapy, the Bacteroides, Bacteroidaceae, Enterococcaceae, and Enterococcus genera showed relative abundance advantages in the group.Conclusion: Traditional Chinese Medicine′s fasting therapy has a significant regulatory effect on the gut microbiota of obese PCOS patients, which may regulate the body′s metabolism by affecting the production of short chain fatty acids.
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基本信息:
DOI：10.16368/j.issn.1674-8999.2026.02.036
中图分类号:R271.9
引用信息:
[1]何孟阳,岳梦锦,查青林,等.中医休粮疗法对肥胖型多囊卵巢综合征患者肠道菌群的影响[J].中医学报,2026,41(02):258-264.DOI:10.16368/j.issn.1674-8999.2026.02.036.
基金信息:
江西省科技厅重点研发计划项目(20171ACG70011)
2025-03-11
2025
2026-03-02
2026
2
2025-09-03
2025-09-03
2025-09-03